The urine is collected over a number of days. The morning portion of urine is disposed of. All consecutive portions of urine excreted throughout the day, night as well as the morning of the next day are collected into a single container. After collection, the urine should be stored in a cool environment. After the final sample of urine is collected, the contents of the container must be accurately measured, stirred and immediately poured into a small jar. The latter should be brought into the medical office for examination. It is not necessary to bring in the whole sample of urine, but the patient must record the daily volume of urine in milliliters.